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세미나 담당교수 : 2022-2학기 김진홍(jinhkim@snu.ac.kr), 이은주(ejlee@snu.ac.kr), Piotr Grzegorz Jablonski (piotrjab@snu.ac.kr)
조 교 : 홍석영(880-4418, young.hong023@snu.ac.kr)
호암교수회관 : 5572, 교수회관: 5241, 두레미담: 9358, 라쿠치나: 1631.

[초청강연] Topology of mammalian transcription

2022-11-15l 조회수 173

일시: 2022-11-18 11:00 ~ 13:00
발표자: Won-Ki Cho (KAIST Dept. of Biological Sciences)
담당교수: 생명과학부
장소: 대면 | 목암홀(Mokam Hall) https://snu-ac-kr.zoom.us/j/95568098277
Topology of mammalian transcription

Won-Ki Cho

KAIST, Department of Biological Sciences

Mammalian genome is organized into a hierarchical structure at multiple scales in a cell nucleus.
At the most macroscopic scale, individual chromosomes are segregated into own separated
intranuclear regions, which is so called chromosome territories. On the other hand, recent
intensive studies using live-cell imaging techniques have characterized behaviors of subnuclear
compartments, exemplified with transcriptional condensates and nuclear speckles. They are
functionally specialized non-membrane bound organelles, mostly follow liquid properties.
However, how the genome structure and subnuclear organelles are spatially organized in the
nucleus has not been elucidated. We found that chromatin looping by CTCF, a chromatin
architectural protein, acts as an architectural prerequisite for the assembly of phase-separated
transcriptional condensates. Moreover, we observed a layered structure of transcriptional and
splicing condensates near the nuclear matrix discovered by super-resolution imaging of RNA
polymerase II, nuclear speckles, and Scaffold attachment factor A (SAF-A, a nuclear matrix
associated proteins).

Key words: Nucleus, Gene expression, Transcription, Chromatin, Transcriptional condensate,
Genome organization, Super-resolution, Live cell imaging