[초청강연] In vivo proximity labeling and beyond

Proximity labeling can be defined as an enzymatic “in-cell” chemical reaction that catalyzes
the proximity-dependent modification of biomolecules in live cells. Since the modified proteins
can be isolated and identified via mass spectrometry, this method has been successfully utilized
for the characterization of local proteomes such as the sub-mitochondrial proteome and the
proteome at membrane contact sites, or spatiotemporal interactome information in live cells,
which are not “accessible” via conventional methods. Currently, proximity labeling techniques
can be applied for local proteome mapping in a specific tissue of animal model which contains
physiological-relevant information. We expect that proximity labeling can be as an essential
tool in “spatiomics”, that is, for the extraction of spatially-distributed biological information in
a cell or organism. In this seminar, I will prseent our current progress of in vivo proximity
labeling research in mitochondrial biology and in tissue-specific secretome profiling. I will also
discuss about further direction of proximity labeling tools for other biological studies.