Kim, V. Narry
Our lab researches gene regulation mediated by RNA. RNAs are at the core of posttranscriptional regulation, and play pivotal roles in developmental and pathological pathways. We currently work on three major topics (microRNA, RNA tail, and RNA binding proteins) applying biochemical and molecular biological tools, as well as genetic, biophysical, and computational approaches.
microRNAs are small noncoding RNAs involved in virtually all functional aspects of cellular function and developmental control, including cell differentiation, proliferation, death, embryogenesis, energy metabolism, and antiviral defense. Tight control of microRNA is critical for normal functioning of cells, and the dysregulation of microRNAs often causes human diseases such as cancer. We made major contributions to the current understanding of how microRNAs are created and processed by elucidating the mechanism of microRNA biogenesis and identifying several key factors in the pathway, including DROSHA. We have also been investigating the function and regulation of microRNAs in cancer and stem cells. We hope that our studies will provide new opportunities for cancer treatment and stem cell engineering.
RNAs are often modified at the 3' end, which is critical for their stabilization, functionalization, and/or degradation. We previously found that some microRNAs are uridylated or adenylated and that the 3' tails regulate the biogenesis and stability of microRNAs. More recently, we developed a technique called TAIL-seq that allows the genomic scale investigation of mRNA tails. Using this new tool, we are studying the function of mRNA tails in the context of development and diseases.
RNAs are always in complex with a selective set of RNA binding proteins (RBPs) that act on all stages of RNA life cycle. We have set up a state-of-the-art proteomic facility, and are developing new tools to investigate the RNA-protein network. This study will help unveil the functions and mechanisms of RNAs in posttranscriptional gene regulation.
We welcome postdoc applications from all around the world and all areas of biology, in particular proteomics, bioinformatics, and structural biology. For more information and inquiries, please email us at narrykim at snu dot ac dot kr.
- S. C. Kwon, T. A. Nguyen, Y.-G. Choi, M. H. Jo, S. Hohng, V. N. Kim* and J.-S. Woo* (2016) “Structure of Human DROSHA” Cell, 164(1-2):81-90. (*co-corresponding authors)
- J. Cho, N.-K. Yu, J.-H. Choi, S.-E. Sim, S. J. Kang, C. Kwak, S.-W. Lee, J. Kim, D. I. Choi, V. N. Kim* and B.-K. Kaang* (2015) "Multiple repressive mechanisms in the hippocampus during memory formation" Science, 350(6256):82–87. (*co-corresponding authors)
- J. Lim, M. Ha, H. Chang, S. C. Kwon, D. K. Simanshu, D. J. Patel, and V. N. Kim (2014) "Uridylation by TUT4 and TUT7 marks mRNA for degradation" Cell, 159(6):1365-1376.
- I. Heo, M. Ha, J. Lim, M.-J. Yoon, J.-E. Park, S. C. Kwon, H. Chang and V. N. Kim (2012) “Mono-uridylation of pre-microRNA as a key step in the biogenesis of group II let-7 microRNAs” Cell, 151:521-532.
- J.-E. Park, I. Heo, Y. Tian, D. K. Simanshu, H. Chang, D. Jee, D. J. Patel and V. N. Kim (2011) "Dicer recognizes the 5' end of RNA for efficient and accurate processing" Nature, 475:201-205.