RNA-binding proteins (RBPs) interact with mRNAs or other RNA molecules to regulate gene expression through various mechanisms in the cell such as splicing and translation. Recently developed technique called crosslinking and immunoprecipitation followed by high-throughput sequencing (CLIP-Seq), and its improved methods such as iCLIP and eCLIP have provided information regarding the RNA and RBP interaction which can be studied at a single nucleotide resolution. Our lab is interested in better understanding gene regulation elicited by the RBPs by utilizing large-scale RBP data provided by iCLIP and eCLIP techniques.
(Flowchart for discovery of RBP functions in omics scale, plot by Sukjun Kim)
(Basic statistics for eCLIP-seq data, plot by Sukjun Kim)