Seoul National University Biological Sciences Website

Publications

Laboratory of Membrane Biology

BBA – General Subjects

Sung-jun Jung, Yunjae Jung, Hyun Kim, "Proper insertion and topogenesis of membrane proteins in the ER depend on Sec63," BBA – General Subjects, 1863(9), 1371-1380(2019).

BBA Biomembranes

Sung-jun Jung, Mekang Yun, Chewon Yim, Sujin Hong, Won-Ki Huh, Hyun Kim, "Expression level of Sec62 modulates membrane insertion of marginally hydrophobic segments," BBA Biomembranes, 1864(), 184051(2022).

BBA-Mol. Cell Res

Chewon Yim, Sung-jun Jung, Ji Eun Hani Kim, Yunjae Jung, Seoyoon Dymphna Jeong and Hyun Kim, "Profiling of signal sequence characteristics and requirement of different translocation components," BBA-Mol. Cell Res, 1865(11), 1640-1648(2018).

Biochem. Biophys. Res. Commun.

Hunsang Lee and Hyun Kim, "Membrane topology of transmembrane proteins: determinants and experimental tools," Biochem. Biophys. Res. Commun., 453(2), 268-276(2014).

Hunsang Lee, Patricia Lara, Angela Ostuni, Jenny Presto, Janne Johansson, IngMarie Nilsson, and Hyun Kim, "Live-cell topology assessment of URG7, MRP6₁₀₂ and SP-C using glycosylatable green fluorescent protein in mammalian cells," Biochem. Biophys. Res. Commun., 450(4), 1587-1592(2014).

Hunsang Lee, Jisoo Min, Gunnar von Heijneb, and Hyun Kim, "Glycosylatable GFP as a compartment-specific membrane topology reporter," Biochem. Biophys. Res. Commun., 427(4), 780-784(2012).

EMBO J.

Salomé Calado Botelho, Marie Osterberg, Andreas S Reichert, Koji Yamano, Patrik Björkholm, Toshiya Endo, Gunnar Von Heijne, and Hyun Kim, "TIM23-mediated insertion of transmembrane α-helices into the mitochondrial inner membrane," EMBO J., 30(6), 1003-1011(2011).

FEBS Lett.

Kwangjin Park, Sung-jun Jung, Hyeonseong Kim, and Hyun Kim, "Mode of membrane insertion of individual transmembrane segments in Mdl1 and Mdl2, multi-spanning mitochondrial ABC transporters," FEBS Lett., 588(18), 3445-3453(2014).

Johannes H. Reithinger, Chewon Yim, Kwangjin Park, Patrik Björkholm, Gunnar von Heijne, and Hyun Kim, "A short C-terminal tail prevents mis-targeting of hydrophobic mitochondrial membrane proteins to the ER," FEBS Lett., 587(21), 3480-3486(2013).

Marie Österberg, Salomé Calado Botelho, Gunnar von Heijne, and Hyun Kim, "Charged flanking residues control the efficiency of membrane insertion of the first transmembrane segment in yeast mitochondrial Mgm1p," FEBS Lett., 585(5), 1238-1242(2011).

FEBS letters

Lee S, Lee H, Yoo S, Leva R, van der Laan M, von Heijne G, Kim H, "The Mgr2 subunit of the TIM23 complex regulates membrane insertion of marginal stop-transfer signals in the mitochondrial inner membrane," FEBS letters, 594(6), 1081-1087(2019).

Genetics

Susanne Kerje, Preety Sharma, Ulrika Gunnarsson, Hyun Kim, Sonchita Bagchi, Robert Fredriksson, Karin Schütz, Per Jensen, Gunnar von Heijne, Ron Okimoto, and Leif Andersson, "The Dominant white, Dun and Smoky color variants in chicken are associated with insertion/deletion polymorphisms in the PMEL17 gene," Genetics, 168(3), 1507-1518(2004).

International Journal of Molecular Sciences

• Sung-jun Jung and Hyun Kim, "Emerging View on the Molecular Functions of Sec62 and Sec63 in Protein Translocation," International Journal of Molecular Sciences, 22(), 12757(2021).

J. Biol. Chem.

Seoeun Lee, Hunsang Lee, Suji Yoo and Hyun Kim, "Molecular insights into the m-AAA protease mediated dislocation of transmembrane helices in the mitochondrial inner membrane," J. Biol. Chem., 292(49), 20058-20066(2017).

Patricia Lara, Karin Öjemalm, Johannes Reithinger, Aurora Holgado, You Maojun, Abdessalem Hammed, Daniel Mattle, Hyun Kim, and IngMarie Nilsson, "Refined topology model of the STT3/Stt3 protein subunit of the oligosaccharyl transferase complex," J. Biol. Chem., 292(27), 11349-11360(2017).

Johannes H. Reithinger, Chewon Yim, Sungmin Kim, Hunsang Lee, and Hyun Kim,, "Structural and functional profiling of the lateral gate of the Sec61 translocon," J. Biol. Chem., 289(22), 15845-15855(2014).

Johannes H. Reithinger, Ji Eun Hani Kim, and Hyun Kim, "Sec62 protein mediates membrane insertion and orientation of moderately hydrophobic signal anchor proteins in the endoplasmic reticulum (ER)," J. Biol. Chem., 288(25), 18058-18067(2013).

Salomé Calado Botelho, Takashi Tatsuta, Gunnar von Heijne, and Hyun Kim, "Dislocation by the m-AAA protease increases the threshold hydrophobicity for retention of transmembrane helices in the inner membrane of yeast mitochondria," J. Biol. Chem., 288(7), 4792-4798(2013).

Kwangjin Park, Salome Calado Botelho, joonki Hong, Marie Osterberg, and Hyun Kim, "Dissecting stop transfer versus conservative sorting pathways for mitochondrial inner membrane proteins in vivo," J. Biol. Chem., 288(3), 1521-1532(2013).

Hyun Kim, Gunnar Von Heijne, and IngMarie Nilsson, "Membrane topology of the STT3 subunit of the oligosaccharyl transferase complex," J. Biol. Chem., 280(21), 20261-20267(2005).

Hyun Kim, Karin Melen, and Gunnar von Heijne, "Topology models for 37 Saccharomyces cerevisiae membrane proteins based on C-terminal reporter fusions and predictions," J. Biol. Chem., 278(12), 10208-10213(2003).

J. Cell Sci.

Yim C, Chung Y, Kim J, Nilsson I, Kim JS, Kim H, "Spc1 regulates the signal peptidase-mediated processing of membrane proteins," J. Cell Sci., 134(13), (2021).

J. Cell. Sci.

Sung-jun Jung, Ji Eun Hani Kim, Johannes H. Reithinger, and Hyun Kim, "The Sec62/Sec63 translocon facilitates the Cout orientation of membrane proteins," J. Cell. Sci., 127(19), 4270-4278(2014).

J. Mol. Biol.

Jung SJ, Kim JEH, Junne T, Spiess M, Kim H, "Cotranslational Targeting and Posttranslational Translocation can Cooperate in Spc3 Topogenesis," J. Mol. Biol., 433(18), 167109(2021).

Tara Hessa, Johannes H. Reithinger, Gunnar von Heijne and Hyun Kim, "Analysis of transmembrane helix integration in the endoplasmic reticulum in S. cerevisiae," J. Mol. Biol., 386(5), 1222-1228(2009).

Methods Mol. Biol.

David Drew and Hyun Kim, "Optimizing Saccharomyces cerevisiae Induction Regimes," Methods Mol. Biol., 866(), 191-195(2012).

David Drew and Hyun Kim, "Large-scale production of membrane proteins in saccharomyces cerevisiae: Using a green fluorescent protein fusion strategy in the production of membrane proteins," Methods Mol. Biol., 866(), 209-216(2012).

David Drew and Hyun Kim, "Screening for high-yielding Saccharomyces cerevisiae clones: using a green fluorescent protein fusion strategy in the production of membrane proteins," Methods Mol. Biol., 866(), 75-86(2012).

David Drew and Hyun Kim, "Preparation of Saccharomyces cerevisiae expression plasmids," Methods Mol. Biol., 866(), 41-46(2012).

Nat. Protoc.

David Drew, Simon Newstead, Yo Sonoda, Hyun Kim, Gunnar von Heijne, and So Iwata, "GFP-based optimization scheme for the overexpression and purification of eukaryotic membrane proteins in Saccharomyces cerevisiae," Nat. Protoc., 3(5), 784-798(2008).

Nature

Tara Hessa, Nadja M. Meindl-Beinker, Andreas Bernsel, Hyun Kim, Yoko Sato, Mirjam Lerch-Bader, IngMarie Nilsson, Stephen H White, and Gunnar Von Heijne, "Molecular code for transmembrane-helix recognition by the Sec61 translocon," Nature, 450(7172), 1026-1030(2007).

Tara Hessa, Hyun Kim, Karl Bihlmaier, Carolina Lundin, Jorrit Boekel, Helena Andersson, IngMarie Nilsson, Stephen H. White, and Gunnar Von Heijne, "Recognition of transmembrane helices by the endoplasmic reticulum translocon," Nature, 433(7024), 377-381(2005).

PNAS

Carolina Lundin, Hyun Kim, IngMarie Nilsson, Stephen H. White, and Gunnar von Heijne, "Molecular code for protein insertion in the endoplasmic reticulum membrane is similar for N(in)-C(out) and N(out)-C(in) transmembrane helices," PNAS, 105(41), 15702-15707(2008).

Mirjam Lerch-Bader, Carolina Lundin, Hyun Kim, IngMarie Nilsson, and Gunnar von Heijne, "Contribution of positively charged flanking residues to the insertion of transmembrane helices into the endoplasmic reticulum," PNAS, 105(11), 4127-4132(2008).

Simon Newstead, Hyun Kim, Gunnar von Heijne, So Iwata, and David Drew, "High-throughput fluorescent-based optimization of eukaryotic membrane protein overexpression and purification in Saccharomyces cerevisiae," PNAS, 104(35), 13936-13941(2007).

Marie Österberg, Hyun Kim, Jonas Warringer, Karin Melén, Anders Blomberg, and Gunnar von Heijne, "Phenotypic effects of membrane protein overexpression in Saccharomyces cerevisiae," PNAS, 103(30), 11148-11153(2006).

Hyun Kim, Karin Melén, Marie Osterberg, and Gunnar Von Heijne, "A global topology map of the Saccharomyces cerevisiae membrane proteome," PNAS, 103(30), 11142-11147(2006).

Hyun Kim, Qi Yan, Gunnar von Heijne, Greg Caputo, and William J. Lennarz, "Determination of the membrane topology of Ost4p and its subunit interactions in the oligosaccharyltransferase complex in Saccharomyces cerevisiae," PNAS, 100(13), 7460-7464(2003).

Hyun Kim, Hangil Park, Lucy Montalvo, and William J. Lennarz, "Studies on the role of the hydrophobic domain of Ost4p in interactions with other subunits of yeast oligosaccharyl transferase," PNAS, 97(4), 1516-1520(2000).

Protein. Sci.

Hayoung Kim, Salomé Calado Botelho, Kwangjin Park, and Hyun Kim, "Use of carbonate extraction in analyzing moderately hydrophobic transmembrane proteins in the mitochondrial inner membrane," Protein. Sci., 24(12), 2063-2069(2015).

Science

Jianhua Ren, Kelli Kachel, Hyun Kim, Susan E.Malenbaum, R.John Collier, and Erwin London, "Interaction of diphtheria toxin T domain with molten globule-like proteins and its implications for translocation," Science, 284(5416), 955-957(1999).