Seoul National University Biological Sciences Website

Publications

Laboratory of Molecular and Cellular Proteomics

ACS Med. Chem. Lett.

Joung, M., Kim, M., Choi, H., Kim, S. W., Kim, G., Aswar, V. R., Baek, S., Kim, H.-T., Huh, W.-K., Jeong L. S. (2025). Discovery of a Novel Template, N 3 1'-Homologated 4'-Truncated Thiosugar-Substituted Xanthine as an A3AR Antagonist via Conversion of an A1AR Antagonist. ACS Med. Chem. Lett., 16(12), 2520-2528.

Joung, M., Choi, H., Kim, M., Kim, S. W., Kim, G., Aswar, V. R., Baek, S., Kim, H.-T., Huh, W.-K., Jeong, L. S. (2025). Design, Synthesis, and Biological Evaluation of C2-(N-Substituted Amino) Truncated 4'-Thioadenosine Derivatives as A2AAR and A3AR Dual Ligands. ACS Med. Chem. Lett., 16(12), 2512-2519.

Aging

Ha, C. W., and Huh, W.-K. (2011). The implication of Sir2 in replicative aging and senescence in Saccharomyces cerevisiae. Aging, 3(3), 319-324.

Anal. Biochem.

Song, Y. B., Park, C. O, Jeong, J.-Y., and Huh, W.-K. (2014). Monitoring G protein-coupled receptor activation using an adenovirus-based beta-arrestin bimolecular fluorescence complementation assay. Anal. Biochem., 449(), 32-41.

Autophagy

Lee, Y., Kim, B., Jang, H.-S., and Huh, W.-K. (2023). Atg1-dependent phosphorylation of Vps34 is required for dynamic regulation of the phagophore assembly site and autophagy in Saccharomyces cerevisiae. Autophagy, 19(9), 2428-2442.

Kim, B., Lee, Y., Choi, H., and Huh, W.-K. (2021). The trehalose-6-phosphate phosphatase Tps2 regulates ATG8 transcription and autophagy in Saccharomyces cerevisiae. Autophagy, 17(4), 1013-1027.

Klionsky, D. J. et al. (2016). Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition). Autophagy, 12(1), 1-222.

Klionsky, D. J. et al. (2012). Guidelines for the use and interpretation of assays for monitoring autophagy. Autophagy, 8(4), 445-544.

Shin, C.-S., and Huh, W.-K. (2011). Bidirectional regulation between TORC1 and autophagy in Saccharomyces cerevisiae. Autophagy, 7(8), 854-862.

Biochem. Biophys. Res. Commun.

Jang, H.-S., Lee, Y., Kim, Y., and Huh, W.-K. (2024). The ubiquitin-proteasome system degrades fatty acid synthase under nitrogen starvation when autophagy is dysfunctional in Saccharomyces cerevisiae. Biochem. Biophys. Res. Commun., 733(), 150423.

Yi, D.-G., and Huh, W.-K. (2015). UDP-glucose pyrophosphorylase Ugp1 is involved in oxidative stress response and long-term survival during stationary phase in Saccharomyces cerevisiae. Biochem. Biophys. Res. Commun., 467(4), 657-663.

Kim, Y., Chay, K.-O., Song, Y. B., Kim, T.-Y., Kim, I., Han, S.-J., Ahn, Y., Cho, S.-H., Hoe, K.-L., Ahn, B. W., Huh, W.-K., and Lee, S.-R. (2011). Redox regulation of the tumor suppressor PTEN by glutaredoxin 5 and Ycp4. Biochem. Biophys. Res. Commun., 407(1), 175–180.

Huh, W.-K., Song, Y. B., Lee, Y.-S., Ha, C. W., Kim, S.-T., and Kang, S.-O. (2008). D-Erythroascorbic acid activates cyanide-resistant respiration in Candida albicans. Biochem. Biophys. Res. Commun., 369(), 401-406.

Biochim. Biophys. Acta

Song, Y. B., Jhun, M. A., Park, T., and Huh, W.-K. (2010). Quantitative proteomic analysis of ribosomal protein L35b mutant of Saccharomyces cerevisiae. Biochim. Biophys. Acta, 1804(4), 676-683.

Biochim. Biophys. Acta, Biomembr.

Jung, S.-J., Yun, M., Yim, C., Hong, S., Huh, W.-K., and Kim, H. (2022). Expression level of Sec62 modulates membrane insertion of marginally hydrophobic segments. Biochim. Biophys. Acta, Biomembr., 1864(12), 184051.

Cell Commun. Signal.

Kim, H.-T., Jeong, J.-Y., and Huh, W.-K. (2025). Regulation of CXCR4 function by S1P1 through heteromerization. Cell Commun. Signal., 23(), 111.

Hong, J. M., Lee, J.-W., Seen, D.-S., Jeong, J.-Y., and Huh, W.-K. (2023). LPA1-mediated inhibition of CXCR4 attenuates CXCL12-induced signaling and cell migration. Cell Commun. Signal., 21(), 257.

Comp. Funct. Genom.

Howson, R., Huh, W.-K., Ghaemmaghami, S., Falvo, J. V., Bower, K., Belle, A., Dephoure, N., Wykoff, D. D., Weissman, J. S., and O’Shea, E. K. (2005). Construction, verification and experimental use of two epitope-tagged collections of budding yeast strains. Comp. Funct. Genom., 6(), 2-16.

FEBS Lett.

Ramesh, R., Dautel, M., Lee, Y., Kim, Y., Storey, K., Gottfried, S., Goss Kinzy, T., Huh, W.-K., and Sattlegger, E. (2021). Asp56 in actin is critical for the full activity of the amino acid starvation-responsive kinase Gcn2. FEBS Lett., 595(14), 1886-1901.

Yi, D.-G., and Huh, W.-K. (2015). PKA, PHO and stress response pathways regulate the expression of UDP-glucose pyrophosphorylase through Msn2/4 in budding yeast. FEBS Lett., 589(18), 2409-2416.

Kim, Y., Song, Y. B., Kim, T.-Y., Kim, I., Han, S.-J., Ahn, Y., Cho, S.-H., Chay, K.-O., Yang, S. Y., Ahn, B. W., Huh, W.-K., and Lee, S.-R. (2010). Redox regulation of the tumor suppressor PTEN by glutathione. FEBS Lett., 584(16), 3550-3556.

Free Radic. Biol. Med.

Yi, D.-G., Kim, M. J., Choi, J. E., Lee, J., Jung, J., Huh, W.-K., and Chung, W.-H. (2016). Yap1 and Skn7 genetically interact with Rad51 in response to oxidative stress and DNA double-strand break in Saccharomyces cerevisiae. Free Radic. Biol. Med., 101(), 424-433.

G3 (Bethesda)

Singh, K., Lee, M. E., Entezari, M., Jung, C.-H., Kim, Y., Park, Y., Fioretti, J. D., Huh, W.-K., Park, H.-O., Kang, P. J. (2019). Genome-wide studies of Rho5-interacting proteins that are involved in oxidant-induced cell death in budding yeast. G3 (Bethesda), 9(3), 921-931.

Genome Biol.

Lee, H.-G., Lee, H.-S., Jeon, S.-H., Chung, T.-H., Lim, Y.-S., and Huh, W.-K. (2008). High-resolution analysis of condition-specific regulatory modules in Saccharomyces cerevisiae. Genome Biol., 9(), R2.

Genome Res.

Kim, Y., Jung, J. P., Pack, C.-G., and Huh, W.-K. (2019). Global analysis of protein homomerization in Saccharomyces cerevisiae. Genome Res., 29(1), 135-145.

Sung, M.-K., Lim, G., Yi, D.-G., Chang, Y. J., Yang, E. B., Lee, K., and Huh, W.-K. (2013). Genome-wide bimolecular fluorescence complementation analysis of SUMO interactome in yeast. Genome Res., 23(4), 736-746.

Genomics & Informatics

Chang, Y. J., Shin, C.-S., Han, D., Kim, J., Kim, K., Kwon, Y.-M., and Huh, W.-K. (2010). Genomewide profiling of rapamycin sensitivity in Saccharomyces cerevisiae on synthetic medium. Genomics & Informatics, 8(4), 177-184.

Shin, C.-S., Chang, Y.-J., Lee, H.-G., and Huh, W.-K. (2009). Rapamycin-Induced Abundance Changes in the Proteome of Budding Yeast. Genomics & Informatics, 7(), 203-207.

J. Biol. Chem.

Hong, S., Lee, H., and Huh, W.-K. (2024). ARV1 deficiency induces lipid bilayer stress and enhances rDNA stability by activating the unfolded protein response in Saccharomyces cerevisiae. J. Biol. Chem., 300(5), 107273.

Hong, S. and Huh, W.-K. (2021). Loss of Smi1, a protein involved in cell wall synthesis, extends replicative lifespan by enhancing rDNA stability in Saccharomyces cerevisiae. J. Biol. Chem., (), DOI: 10.1074/jbc.RA120.015456.

J. Biotechnol.

Choi, E.-W., Seen, D.-S., Song, Y. B., Son, H.-S., Jung, N.-C., Huh, W.-K., Hahn, J.-S., Kim, K., Jeong, J.-Y., and Lee, T. G. (2012). AdHTS: A high-throughput system for generating recombinant adenoviruses. J. Biotechnol., 162(2-3), 246-252.

J. Cell Biol.

Chang, Y., Lim, G., and Huh, W.-K. (2021). Analysis of the TORC1 interactome reveals spatially distinct function of TORC1 in mRNP complexes. J. Cell Biol., 220(4), e201912060.

Sing, T., Hung, M. P., Ohnuki, S., Suzuki G., San Luis, B.-J., McClain, M., Unruh, J., Yu, Z., Ou, J., Marshall-Sheppard, J., Huh, W.-K., Costanzo, M., Boone, C., Ohya, Y., Jaspersen, S., and Brown, G. (2018). The budding yeast RSC complex maintains ploidy by promoting spindle pole body insertion. J. Cell Biol., 217(7), 2445-2462.

J. Cell Sci.

Shin, C.-S., Kim, S. Y., and Huh, W.-K. (2009). TORC1 Controls Degradation of Transcription Factor Stp1, a Key Effector of the SPS Amino Acid-Sensing Pathway in Saccharomyces cerevisiae. J. Cell Sci., 122(), 2089-2099.

J. Immunol.

Li, H.-M., Jang, J. H., Jung, J.-S., Shin, J., Park, C. O., Kim, Y.-J., Ahn, W.-G., Nam, J.-S., Hong, C.-W., Lee, J., Jung, Y.-J., Chen, J.-F., Ravid, K., Lee, H. T., Huh, W.-K., Kabarowski, J. H., and Song, D.-K. (2019). G2A protects mice against sepsis by modulating Kupffer cell activation: Cooperativity with adenosine receptor 2b. J. Immunol., 202(2), 527-538.

J. Microbiol. Biotechnol.

So, Y. S., Yang, D. H., Jung, K. W., Huh, W.-K., and Bahn, Y. S. (2017). Molecular characterization of adenylyl cyclase complex proteins using versatile protein-tagging plasmid systems in Cryptococcus neoformans. J. Microbiol. Biotechnol., 27(2), 357-364.

J. Microbiol. Methods

Sung, M.-K., and Huh, W.-K. (2010). In vivo quantification of protein-protein interactions in Saccharomyces cerevisiae using bimolecular fluorescence complementation assay. J. Microbiol. Methods, 83(2), 194–201.

J. Mol. Biol.

Miller, K. E., Kim, Y., Huh, W.-K., and Park, H.-O. (2015). Bimolecular fluorescence complementation (BiFC) analysis: advances and recent applications for genome-wide interaction studies. J. Mol. Biol., 427(11), 2039-2055.

Microbiol. Spectr.

Hong , S., Kim, S.-K., Chung, C. H., Yun, C.-H., Lee, J., Cho, C.-S., and Huh, W.-K. (2024). Pullulan nanoparticles inhibit the pathogenicity of Candida albicans by regulating hypha-related gene expression. Microbiol. Spectr., 12(12), e0104824.

Mol. Cells

Kim, T.-Y., Ha, C. W., and Huh, W.-K. (2009). Differential Subcellular Localization of Ribosomal Protein L7 Paralogs in Saccharomyces cerevisiae. Mol. Cells, 27(), 539-546.

Mol. Microbiol.

Lee, P., Paik, S. M., Shin, C. S., Huh, W.-K., and Hahn, J. S. (2011). Regulation of yeast Yak1 kinase by PKA and autophosphorylation-dependent 14-3-3 binding. Mol. Microbiol., 79(3), 633-646.

Nat. Commun.

Choi, C., Bae, J., Kim, S., Lee, S., Kang, H., Kim, J., Bang, I., Kim, K., Huh, W.-K., Seok, C., Park, H., Im, W., Choi, H.J. (2023). Understanding the molecular mechanisms of odorant binding and activation of the human OR52 family. Nat. Commun., 14(1), 8105.

Nature

Huh, W.-K., Falvo, J., Gerke, L., Carroll, A., Howson, R., Weissman, J. S., and O'Shea, E. K. (2003). Global analysis of protein localization in budding yeast. Nature, 425(), 686-691.

Ghaemmaghami, S., Huh, W.-K., Bower, K., Howson, R., Belle, A., Dephoure, N., O'Shea, E. K., and Weissman, J. S. (2003). Global analysis of protein expression in yeast. Nature, 425(), 737-741.

Nucleic Acids Res.

Chang, Y., and Huh, W.-K. (2018). Ksp1-dependent phosphorylation of eIF4G modulates post-transcriptional regulation of specific mRNAs under glucose deprivation conditions. Nucleic Acids Res., 46(6), 3047-3060.

Ha, C. W., Kim, K., Chang, Y. J., Kim, B., and Huh, W.-K. (2014). The β-1,3-glucanosyltransferase Gas1 regulates Sir2-mediated rDNA stability in Saccharomyces cerevisiae. Nucleic Acids Res., 42(13), 8486-8499.

Ha, C. W., Sung, M.-K., and Huh, W.-K. (2012). Nsi1 plays a significant role in the silencing of ribosomal DNA in Saccharomyces cerevisiae. Nucleic Acids Res., 40(11), 4892-4903.

Ha, C. W., and Huh, W.-K. (2011). Rapamycin increases rDNA stability by enhancing association of Sir2 with rDNA in Saccharomyces cerevisiae. Nucleic Acids Res., 39(4), 1336-1350.

Lee, K. Y., Chuang, H.-Y., Beyer, A., Sung, M.-K., Huh, W.-K., Lee, B., and Ideker, T. (2008). Protein networks markedly improve prediction of subcellular localization in multiple eukaryotic species. Nucleic Acids Res., 36(), e136.

PLoS One

Sukhtankar, D. D., Fung, J. J., Kim, M-n., Cayton, T., Chiou, V., Caculitan, N. G., Zalicki, P., Kim, S., Jo, Y., Kim, S., Lee, J. M., Choi, J., Mun, S., Chin, A., Jang, Y., Lee, J. Y., Kim, G., Kim, E. H., Huh, W.-K., Jeong, J.-Y., Seen, D.-S., Cardarelli, P. M. (2023). GPC-100, a novel CXCR4 antagonist, improves in vivo hematopoietic cell mobilization when combined with propranolol. PLoS One, 18(10), e0287863.

Yi, D.-G., Hong, S., and Huh, W.-K. (2018). Mitochondrial dysfunction reduces yeast replicative lifespan by elevating RAS-dependent ROS production by the ER-localized NADPH oxidase Yno1. PLoS One, 13(6), e0198619.

Proc. Natl. Acad. Sci. U. S. A.

Liang, J., Seghiri, M., Singh, P. K., Seo, H. G., Lee, J. Y., Jo, Y., Song, Y. B., Park, C., Zalicki, P., Jeong, J.-Y., Huh, W.-K., Caculitan, N. G., Smith, A. W. (2024). The β2-adrenergic receptor associates with CXCR4 multimers in human cancer cells. Proc. Natl. Acad. Sci. U. S. A., 121(14), e2304897121.

Proc. Natl. Acad. Sci. U.S.A.

Choi, J.-H., Jeong, Y.-M., Kim, S., Lee, B., Ariyasiri K., Kim, H.-T., Jung, S.-H., Hwang, K.-S., Choi, T.-I., Park, C. O, Huh, W.-K., Carl, M., Rosenfeld, J. A., Raskin, S., Ma, A., Gecz, J., Kim, H.-G., Kim, J.-S., Shin, H.-C., Park, D.-S., Gerlai, R., Jamieson, B. B., Kim, J. S., Iremonger, K. J., Lee, S. H., Shin, H.-S., and Kim, C.-H. (2018). Targeted knockout of a chemokine-like gene increases anxiety and fear responses. Proc. Natl. Acad. Sci. U.S.A., 115(5), E1041-E1050.

Lim, G., and Huh, W.-K. (2017). Rad52 phosphorylation by Ipl1 and Mps1 contributes to Mps1 kinetochore localization and spindle assembly checkpoint regulation. Proc. Natl. Acad. Sci. U.S.A., 114(44), E9261-E9270.

Lee, K., Sung, M.-K., Kim, J., Kim, K., Paik, H., Kim, B., Huh, W.-K., and Ideker, T. (2014). Proteome-wide remodeling of protein location and function by stress. Proc. Natl. Acad. Sci. U.S.A., 111(30), E3157-E3166.

Protein & Cell

Pu, J., Ha, C. W., Zhang, S., Jung, J. P., Huh, W.-K., and Liu, P. (2011). Interactomic study on interaction between lipid droplets and mitochondria. Protein & Cell, 2(6), 487-496.

Sci. Adv.

Lim, G., Chang, Y., and Huh, W.-K. (2020). Phosphoregulation of Rad51/Rad52 by CDK1 functions as a molecular switch for cell cycle-specific activation of homologous recombination. Sci. Adv., 6(6), eaay2669.

Sci. Rep.

Park, C., Lee, J.-W., Kim, K., Seen, D.-S., Jeong, J.-Y., and Huh, W.-K. (2023). Simultaneous activation of CXC chemokine receptor 4 and histamine receptor H1 enhances calcium signaling and cancer cell migration. Sci. Rep., 13(), 1894.

Science

Bandyopadhyay, S., Mehta, M., Kuo, D., Sung, M.-K., et al. (2010). Rewiring of genetic networks in response to DNA damage. Science, 330(6009), 1385-1389.

Vaccine

Lee, J.-W., Lee, D.-Y., Baek, S., Jeong, J.-Y., and Huh, W.-K. (2025). Sensitive detection of pertussis toxin in acellular pertussis vaccines using HRH1-mediated calcium signaling. Vaccine, 54(), 127056.

Yeast

Sung, M.-K., Ha, C. W., and Huh, W.-K. (2008). A vector system for efficient and economical switching of C-terminal epitope tags in Saccharomyces cerevisiae. Yeast, 25(), 301-311.

Sung, M.-K., and Huh, W.-K. (2007). Bimolecular fluorescence complementation analysis system for in vivo detection of protein-protein interaction in Saccharomyces cerevisiae. Yeast, 24(), 767-775.

Lee, M.-W., Kim, B.-J., Choi, H.-K., Ryu, M.-J., Kim, S.-B., Kang, K.-M., Cho, E.-J., Youn, H.-D., Huh, W.-K., and Kim, S.-T. (2007). Global protein expression profiling of budding yeast in response to DNA damage. Yeast, 24(), 145-154.

대한민국 특허 (등록번호 10-1875825, 등록일자 2018.07.02)

허원기, 김연수 (2018). 황색 형광 단백질 절편 부착 효모 균주 유전자 라이브러리 및 이를 이용한 단백질 상호작용 분석 방법. 대한민국 특허 (등록번호 10-1875825, 등록일자 2018.07.02), (), .

대한민국 특허 (등록번호 10-2062744, 등록일자 2019.12.30)

허원기, 장연지 (2019). 효모에서 mRNA의 전사후 과정을 조절하는 방법. 대한민국 특허 (등록번호 10-2062744, 등록일자 2019.12.30), (), .

대한민국 특허 (출원번호 10-2006-0096709, 출원일자 2006.09.30; 등록번호 10-0825290, 등록일자 2008.04.21)

허원기, 성민경 (2006). 염색체내 형광단백질 유전자 일부가 삽입된 세포를 포함하는 이분자 형광 상보 시스템 및 이를 이용한 이분자 형광 상보 기법. 대한민국 특허 (출원번호 10-2006-0096709, 출원일자 2006.09.30; 등록번호 10-0825290, 등록일자 2008.04.21), (), .

대한민국 특허 (출원번호 10-2008-0003443, 출원일자 2008.01.11; 등록번호 10-0927098, 등록일자 2009.11.09)

허원기, 성민경 (2008). 에피토프 태그 치환용 재조합 벡터. 대한민국 특허 (출원번호 10-2008-0003443, 출원일자 2008.01.11; 등록번호 10-0927098, 등록일자 2009.11.09), (), .

대한민국 특허 (출원번호 10-2008-0094298, 출원일자 2008.09.25; 등록번호 10-1050669, 등록일자 2011.07.13)

허원기, 성민경 (2008). 이분자 형광 상보 기법을 이용하여 단백질의 수모화를 분석하는 방법 및 그에 사용되는 벡터들. 대한민국 특허 (출원번호 10-2008-0094298, 출원일자 2008.09.25; 등록번호 10-1050669, 등록일자 2011.07.13), (), .

대한민국 특허 (출원번호 10-2009-0048746, 출원일자 2009.06.02; 등록번호 10-1139589, 등록일자 2012.04.17)

허원기, 성민경, 양은빈, 이대관, 임규범 (2009). 생체 내 단백질 상호작용 분석을 위한 황색형광단백질 N-말단 절편 부착 효모 균주 라이브러리. 대한민국 특허 (출원번호 10-2009-0048746, 출원일자 2009.06.02; 등록번호 10-1139589, 등록일자 2012.04.17), (), .

대한민국 특허 (출원번호 10-2010-0058213, 출원일자 2010.06.18; 등록번호 10-1029972, 등록일자 2011.04.12)

허원기, 송용범, 이태규, 신동승, 정재연, 최은욱, 안지선 (2010). 바이러스 벡터를 이용하여 단백질의 상호작용을 측정하는 방법. 대한민국 특허 (출원번호 10-2010-0058213, 출원일자 2010.06.18; 등록번호 10-1029972, 등록일자 2011.04.12), (), .

대한민국 특허 (출원번호 10-2017-0022508, 출원일자 2017.02.20)

허원기, 김연수 (2017). 황색 형광 단백질 절편 부착 효모 균주 유전자 라이브러리 및 이를 이용한 단백질 상호작용 분석 방법. 대한민국 특허 (출원번호 10-2017-0022508, 출원일자 2017.02.20), (), .

대한민국 특허 (출원번호 10-2017-0181546, 출원일자 2017.12.27)

허원기, 장연지 (2017). 효모에서 mRNA의 전사후 과정을 조절하는 방법. 대한민국 특허 (출원번호 10-2017-0181546, 출원일자 2017.12.27), (), .

대한민국 특허 (출원번호 10-2023-0145748, 출원일자 2023.10.27)

허원기, 조종수, 홍수진 (2023). 다당류 나노입자를 포함하는 칸디다 바이오필름 억제제 및 칸디다증 예방 또는 치료용 조성물. 대한민국 특허 (출원번호 10-2023-0145748, 출원일자 2023.10.27), (), .

대한민국 특허 (출원번호 10-2025-0031434, 출원일자 2025.03.11)

허원기, 정재연, 이진우 (2025). 백일해 독소의 검출 방법. 대한민국 특허 (출원번호 10-2025-0031434, 출원일자 2025.03.11), (), .