HCMV, a member of herpesvirus, expresses several proteins immediately after infection. Two of those proteins, IE1 and IE2, are expressed from the same promoter by differential splicing, but their genetic and biochemical properties appear to be very different. IE1 activates specific transcription factors, while IE2 acts on the general transcription machinery, activating a variety of promoters including TATA-less promoters. The study of these proteins provides an excellent model for understanding the interaction between the host cells and the virus. We have constructed retroviral vectors expressing IE and IE2, separately or simultaneously, and have established related subcloned permanent cell lines. Using DNA chips, the expression profile of human genes was analyzed. The most significantly affected genes, negatively or positively, include p53, chaperon proteins, some structural proteins, and anti-angiogenic genes. Our data strongly suggest specific roles of IE1 and IE2 in certain types of human tumors.
Laboratory of Virology
SEOUL NATIONAL UNIVERSITY BIOLOGICAL SCIENCES