IntroductoryAfter the invention of PCR in 1980s, the Next Generation Sequencing (NGS) platform became the most significant invention in scientific research institutions. It provides huge amounts of sequencing data compared to any other sequencing platforms. However, professional computing skills, Bioinformatics skills, are needed in order to process and analyze big data. Not only computing skills but also knowledge of Biology is required to find meaningful analysis and interpretation of sequencing data. The research in our laboratory is focused on issues in today’s medical and biological field by producing and analyzing NGS data. Moreover, we challenge to invent software and database that can assist in analyzing data. We believe that it would be the most rewarding if we can contribute others’ research in science field by providing our software. Details of our research 1. Microbial diversity and evolutionMicroorganisms went through evolution for more than 3 billion years to have today’s genetic variety, but mankind only started to understand this by virtue of NGS technology.