Yestersay,

 1.I contined the RT PCR,  The day before yesterday I did PCR  only once with UBQ and then with CYP85A2 primers just to check whether BL treatment did work or not.Yesterday I was doing PCR with UBQ to get loading control condition I got the amplification but in 2nd and 3rd try suddenly I could not get PCR amplification. So  today I will try again by changing taq buffer etc.

2.   sowed the seeds of F2 of gul4XBES1:GFP.BES1:GFPX gul4, DR5:GUSX gul4, bin2-1DXgul4, bri1-5XDR5:GUS, bin2-1DXDR5:GUS,dwf4-1XDR5:GUS and F1gul4Xbzr1-D and controll line for all crosses.

3. I took the GUS staining photo if F2  of gul4X p6-1 selected in Kan media and T2 of at4g31250 :GUS selected in Basta. The staining in gul4:DWF4pro:GUS is a bit strange, there is no staining in root tip as in p6-1 but dark staining in the upper part of the root, maturation zone . Regarding the T2 of at4g31250:GUS lins the staining is in the maturation zone.

 I will send the photo by email.

Today I will contine the RT PCR.

gDNA extraction and PCR to confirm the double mutatn in F2 population of gul4Xdwf4-1 .