Puna Maya Maharjanl 2011-02-01l Hit 593
Yesterday,
I prepared the tissue powder of T2 of 35S:CYP83B1:YFP:HA in Ws-2 abckground and did western of them as well as T2 of 35S:CYP83B1:YFP:HA in gul4 backgrounds. I did finish staining with primary antibody HA and today I will continue with secondary antibody and detection.
I did agro colony PCR and 3 ml agro culture of 35S:gul4 cDNA:YFP:HA.
I would like to attach the ppt with photo of F1 phenotype of gul4 double mutant.
Today
I will continue western blot.
PCR to confirm the transformation of CYP83B1promoter gDNA:GUS in gul4 and ws-2 background and will take photo.
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