Puna Maya Maharjanl 2011-01-31l Hit 558
Professor,
T1 of CYP83B1 promoter gDNA :GUS and CYP83B1 promoter gDNA :HA are growing. I will take photo of them and send to you.
Regarding gul4 version cloning, today i will do 3 ml culture of agro of 35S:gul4CDS:YFP:HA and thinking to do transformation in thins week in Ws-2 and gul4 as well as in SALK mutant of CYP83B1 may be on later because they are too small and i have just 3 plants in each of 3 line of salk mutant and they may flower very late as in atr4-1 and atr4-2.
------ Original Message ------ Subject :phenotype of of of double mutant of gul4 Posted by :Puna Maya Maharjan Date :2011-01-31 Last day I repeated RT PCR of CYP83B1 in T2 of 35S:CYP83B1:YFP:HA in gul4 backgrounds. I harvested the seeds of F1 of gul4 Xatr4-1. I observed the F1 phenotype of gul4 crossed with yucca 1-D, bes1-D,dwf7-1 and msg2-2D and transplanted into the soil. Today, I will prepare the tissue powder of T2 of 35S:CYP83B1:YFP:HA in Ws-2 abckground and run SDS PAGE of them as well as T2 of 35S:CYP83B1:YFP:HA in gul4 backgrounds. |