phyB lamina bending and BIN2 sequencing
Professor,
- Last week I obtain a band in my PCR for cloning BIN2, and made gel purication and again amplified that product by PCR. But still, since I have non specific band at the beginning I want to ensure that my product is BIN2 so I will send that product for sequencing just with one primer. Just to be sure that is my fragment before continue cloning.
- Today I also need to transfer the rice seeds to agar to grow them for the lamina assay. This time I am not just repeating JMJ703, but also trying with phyB seeds.
- Last week I obtain a band in my PCR for cloning BIN2, and made gel purication and again amplified that product by PCR. But still, since I have non specific band at the beginning I want to ensure that my product is BIN2 so I will send that product for sequencing just with one primer. Just to be sure that is my fragment before continue cloning.
- Today I also need to transfer the rice seeds to agar to grow them for the lamina assay. This time I am not just repeating JMJ703, but also trying with phyB seeds.