phenotype of of of double mutant of gul4

Last day I  repeated RT PCR of CYP83B1 in T2 of 35S:CYP83B1:YFP:HA in gul4 backgrounds.

I harvested the seeds of F1 of gul4 Xatr4-1.

I observed the F1 phenotype of gul4 crossed with yucca 1-D, bes1-D,dwf7-1 and msg2-2D and transplanted into the soil.

 Today,

I will prepare the tissue powder of T2 of 35S:CYP83B1:YFP:HA in Ws-2 abckground and run SDS PAGE of them as well as T2 of 35S:CYP83B1:YFP:HA in gul4 backgrounds.