Agro transformation of 35S:gul4:YFP
Yes professor, Soren Bak group did the detail study of transcriptomiesand metabolomes in knockout line of CYP83B1. Here is the article about it.
------ Original Message ------ Subject :Agro transformation of 35S:gul4:YFP Posted by :Puna Maya Maharjan Date :2011-01-25 Yesterday 1. MIni prep and restriction enzyme reaction and agro transformation of expression clone of gul4 CDS:YFP:HA was done. 2. The tissue of BRZ dose response treatment (0, 10-9,10-8, 10-7, 10-6 M) treated for 24 hours was collected since I found in some literature that the short term treatment of BRZ has no effect in mRNA level of biosynthetic genes. 3. Phenotype of T1 of CYP83B1 :pEarlyGate 301-HA was onserved and photographed 4.Sequence analysis of entry clone of gul4 promoter construct. Today 1. Tissue grinding and western of T2 ofCYP83 B1 OX lines in Ws-2 and gul4. |
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