Yesterday

1. MIni prep and restriction  enzyme reaction and agro transformation of expression clone of gul4 CDS:YFP:HA was done.

2. The tissue of BRZ dose response treatment (0, 10-9,10-8, 10-7, 10-6 M) treated for 24 hours  was collected since I found in some literature that the short term treatment of BRZ has no effect in mRNA level of biosynthetic genes.

3. Phenotype of T1 of CYP83B1 :pEarlyGate 301-HA was onserved and photographed

4.Sequence analysis of entry clone of gul4 promoter construct.

Today

1. Tissue grinding and western of T2 ofCYP83 B1 OX lines in Ws-2 and gul4.