gul4 Double mutant , gul4 allelic variation
Dear Professor,
Last day I did, take photograph of 5 weeks old gul4, atr4-1,and atr4-2. I could not upload the photo now due to some problem in my computer.I will upload as soon as i can operate my computer.
I tried to do dna extraction PCR genotyping to know the double mutatns homo lines of gul4 and dwf4. But i failed to get pcr condition.
Today's plan
1. LR cloning of gul4 CDS entry into pEarlyGate 101-HA.
2. Western blot of BL treated gul4 and Ws-2.
3. Transplantation of F2 of double mutant of gul4 and dwf4 and bri1-5.
4. Miniprep of Entry clone of gul4 promoter construct