Lab Board

gul4 Double mutant , gul4 allelic  variation

Puna Maya Maharjanl 2011-01-17l Hit 592

 Dear Professor,

 Last day I did, take photograph of 5 weeks old gul4, atr4-1,and atr4-2. I could not upload the photo now due to some problem in my computer.I will upload as soon as i can operate my computer.

 I tried to do dna extraction PCR genotyping to know the double mutatns homo lines of gul4 and dwf4. But i failed to get pcr condition.


 Today's plan


1. LR cloning of gul4 CDS entry into pEarlyGate 101-HA.

2. Western blot of BL treated gul4 and Ws-2.

3. Transplantation of F2 of double mutant of gul4 and dwf4 and bri1-5.

4. Miniprep of Entry clone of gul4 promoter construct