01/14 Puna
Puna
Summarize the progress of yesterday, too. Be oriented....
------ Original Message ------ Subject :01/14 Puna Posted by :Puna Maya Maharjan Date :2011-01-14 1. Sequencing results analysis of Entry clone of gul4 CDS, today I will do LR cloning. 2. Seed sowing of the T2 of OX lines of GUL4 in ws-2 and gul4 background in Basta. 3. Transformation confirmation of GUL4 promoter:HA in gul4 background. 4. F2 phenotype analysis and photo taking of double mutant of gul4 with p6-1, dwf4-1 and bri1-5. 5. PCR and cloning of gul4 promoter into pENTR SD/D TOPO. |