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세미나 담당교수 : 2021-1학기 임영운(ywlim@snu.ac.kr), 황대희(daehee@snu.ac.kr), 최명환(choim@snu.ac.kr)
조 교 : 윤창규(880-8827, yoon891023@snu.ac.kr)
호암교수회관 : 5572, 교수회관: 5241, 두레미담: 9358, 라쿠치나: 1631.

[초청강연] 현대생물학 콜로퀴움 - Mass spectrometry based macromolecular interactomics

2021-04-26l 조회수 108

일시: 2021-04-26 09:00 ~ 09:00
발표자: Prof. Jong-Seo Kim (School of Biological Sciences, SNU)
담당교수: 윤창규 학생조교
장소: https://snu-ac-kr.zoom.us/j/83447823367
Mass spectrometry based macromolecular interactomics

Jong-Seo Kim
School of Biological Sciences, Seoul National University

Mass spectrometric profiling of biomolecular interactions such as protein to protein or protein to nucleic acids provides critical information for function, yet is often difficult to accurately capture and define in system-wide manner. To do so, the innate limitation of mass spectrometry compared to next generation sequencing approaches, which is the incapability of molecular amplification via such as PCR, needs to be overcome. In this regard, the analytical development for ultrasensitive and unbiased quantification platform is highly desirable for the next level of molecular interactomics, i.e., system-wide profiling of molecular interactions. Here, I will introduce my recent development for ultrasensitive liquid chromatography and mass spectrometry (LC-MS) system and accurate proteome quantification platform to capture molecular interactions more comprehensively. And then I will show detail examples about the profiling of macromolecular interactions including protein-protein interactions using proximity labeling and RNA-protein interactions, which were enabled by the developed sensitive proteomics platform. Lastly, the future direction toward next generation molecular interactomics including the direct identification of ligands bound to protein via mass spectrometry will be discussed.