Seoul National University Biological Sciences Website

논문

Laboratory of Molecular and Cellular Proteomics

2024

Hong, S., Lee, H., and Huh, W.-K. (2024). ARV1 deficiency induces lipid bilayer stress and enhances rDNA stability by activating the unfolded protein response in Saccharomyces cerevisiae. J. Biol. Chem., (In press), .

Liang, J., Seghiri, M., Singh, P. K., Seo, H. G., Lee, J. Y., Jo, Y., Song, Y. B., Park, C., Zalicki, P., Jeong, J.-Y., Huh, W.-K., Caculitan, N. G., Smith, A. W. (2024). The β2-adrenergic receptor associates with CXCR4 multimers in human cancer cells. Proc. Natl. Acad. Sci. U. S. A., 121(14), e2304897121.

2023

Choi, C., Bae, J., Kim, S., Lee, S., Kang, H., Kim, J., Bang, I., Kim, K., Huh, W.-K., Seok, C., Park, H., Im, W., Choi, H.J. (2023). Understanding the molecular mechanisms of odorant binding and activation of the human OR52 family. Nat. Commun., 14(1), 8105.

허원기, 조종수, 홍수진 (2023). 다당류 나노입자를 포함하는 칸디다 바이오필름 억제제 및 칸디다증 예방 또는 치료용 조성물. 대한민국 특허 (출원번호 10-2023-0145748, 출원일자 2023.10.27), (), .

Sukhtankar, D. D., Fung, J. J., Kim, M-n., Cayton, T., Chiou, V., Caculitan, N. G., Zalicki, P., Kim, S., Jo, Y., Kim, S., Lee, J. M., Choi, J., Mun, S., Chin, A., Jang, Y., Lee, J. Y., Kim, G., Kim, E. H., Huh, W.-K., Jeong, J.-Y., Seen, D.-S., Cardarelli, P. M. (2023). GPC-100, a novel CXCR4 antagonist, improves in vivo hematopoietic cell mobilization when combined with propranolol. PLoS One, 18(10), e0287863.

Hong, J. M., Lee, J.-W., Seen, D.-S., Jeong, J.-Y., and Huh, W.-K. (2023). LPA1-mediated inhibition of CXCR4 attenuates CXCL12-induced signaling and cell migration. Cell Commun. Signal., 21(), 257.

Lee, Y., Kim, B., Jang, H.-S., and Huh, W.-K. (2023). Atg1-dependent phosphorylation of Vps34 is required for dynamic regulation of the phagophore assembly site and autophagy in Saccharomyces cerevisiae. Autophagy, 19(9), 2428-2442.

Park, C., Lee, J.-W., Kim, K., Seen, D.-S., Jeong, J.-Y., and Huh, W.-K. (2023). Simultaneous activation of CXC chemokine receptor 4 and histamine receptor H1 enhances calcium signaling and cancer cell migration. Sci. Rep., 13(), 1894.

2022

Jung, S.-J., Yun, M., Yim, C., Hong, S., Huh, W.-K., and Kim, H. (2022). Expression level of Sec62 modulates membrane insertion of marginally hydrophobic segments. Biochim. Biophys. Acta, Biomembr., 1864(12), 184051.

2021

Ramesh, R., Dautel, M., Lee, Y., Kim, Y., Storey, K., Gottfried, S., Goss Kinzy, T., Huh, W.-K., and Sattlegger, E. (2021). Asp56 in actin is critical for the full activity of the amino acid starvation-responsive kinase Gcn2. FEBS Lett., 595(14), 1886-1901.

Chang, Y., Lim, G., and Huh, W.-K. (2021). Analysis of the TORC1 interactome reveals spatially distinct function of TORC1 in mRNP complexes. J. Cell Biol., 220(4), e201912060.

Kim, B., Lee, Y., Choi, H., and Huh, W.-K. (2021). The trehalose-6-phosphate phosphatase Tps2 regulates ATG8 transcription and autophagy in Saccharomyces cerevisiae. Autophagy, 17(4), 1013-1027.

Hong, S. and Huh, W.-K. (2021). Loss of Smi1, a protein involved in cell wall synthesis, extends replicative lifespan by enhancing rDNA stability in Saccharomyces cerevisiae. J. Biol. Chem., (), DOI: 10.1074/jbc.RA120.015456.

2020

Lim, G., Chang, Y., and Huh, W.-K. (2020). Phosphoregulation of Rad51/Rad52 by CDK1 functions as a molecular switch for cell cycle-specific activation of homologous recombination. Sci. Adv., 6(6), eaay2669.

2019

허원기, 장연지 (2019). 효모에서 mRNA의 전사후 과정을 조절하는 방법. 대한민국 특허 (등록번호 10-2062744, 등록일자 2019.12.30), (), .

Singh, K., Lee, M. E., Entezari, M., Jung, C.-H., Kim, Y., Park, Y., Fioretti, J. D., Huh, W.-K., Park, H.-O., Kang, P. J. (2019). Genome-wide studies of Rho5-interacting proteins that are involved in oxidant-induced cell death in budding yeast. G3 (Bethesda), 9(3), 921-931.

Kim, Y., Jung, J. P., Pack, C.-G., and Huh, W.-K. (2019). Global analysis of protein homomerization in Saccharomyces cerevisiae. Genome Res., 29(1), 135-145.

Li, H.-M., Jang, J. H., Jung, J.-S., Shin, J., Park, C. O., Kim, Y.-J., Ahn, W.-G., Nam, J.-S., Hong, C.-W., Lee, J., Jung, Y.-J., Chen, J.-F., Ravid, K., Lee, H. T., Huh, W.-K., Kabarowski, J. H., and Song, D.-K. (2019). G2A protects mice against sepsis by modulating Kupffer cell activation: Cooperativity with adenosine receptor 2b. J. Immunol., 202(2), 527-538.

2018

허원기, 김연수 (2018). 황색 형광 단백질 절편 부착 효모 균주 유전자 라이브러리 및 이를 이용한 단백질 상호작용 분석 방법. 대한민국 특허 (등록번호 10-1875825, 등록일자 2018.07.02), (), .

Yi, D.-G., Hong, S., and Huh, W.-K. (2018). Mitochondrial dysfunction reduces yeast replicative lifespan by elevating RAS-dependent ROS production by the ER-localized NADPH oxidase Yno1. PLoS One, 13(6), e0198619.

Sing, T., Hung, M. P., Ohnuki, S., Suzuki G., San Luis, B.-J., McClain, M., Unruh, J., Yu, Z., Ou, J., Marshall-Sheppard, J., Huh, W.-K., Costanzo, M., Boone, C., Ohya, Y., Jaspersen, S., and Brown, G. (2018). The budding yeast RSC complex maintains ploidy by promoting spindle pole body insertion. J. Cell Biol., 217(7), 2445-2462.

Chang, Y., and Huh, W.-K. (2018). Ksp1-dependent phosphorylation of eIF4G modulates post-transcriptional regulation of specific mRNAs under glucose deprivation conditions. Nucleic Acids Res., 46(6), 3047-3060.

Choi, J.-H., Jeong, Y.-M., Kim, S., Lee, B., Ariyasiri K., Kim, H.-T., Jung, S.-H., Hwang, K.-S., Choi, T.-I., Park, C. O, Huh, W.-K., Carl, M., Rosenfeld, J. A., Raskin, S., Ma, A., Gecz, J., Kim, H.-G., Kim, J.-S., Shin, H.-C., Park, D.-S., Gerlai, R., Jamieson, B. B., Kim, J. S., Iremonger, K. J., Lee, S. H., Shin, H.-S., and Kim, C.-H. (2018). Targeted knockout of a chemokine-like gene increases anxiety and fear responses. Proc. Natl. Acad. Sci. U.S.A., 115(5), E1041-E1050.

2017

허원기, 장연지 (2017). 효모에서 mRNA의 전사후 과정을 조절하는 방법. 대한민국 특허 (출원번호 10-2017-0181546, 출원일자 2017.12.27), (), .

Lim, G., and Huh, W.-K. (2017). Rad52 phosphorylation by Ipl1 and Mps1 contributes to Mps1 kinetochore localization and spindle assembly checkpoint regulation. Proc. Natl. Acad. Sci. U.S.A., 114(44), E9261-E9270.

허원기, 김연수 (2017). 황색 형광 단백질 절편 부착 효모 균주 유전자 라이브러리 및 이를 이용한 단백질 상호작용 분석 방법. 대한민국 특허 (출원번호 10-2017-0022508, 출원일자 2017.02.20), (), .

So, Y. S., Yang, D. H., Jung, K. W., Huh, W.-K., and Bahn, Y. S. (2017). Molecular characterization of adenylyl cyclase complex proteins using versatile protein-tagging plasmid systems in Cryptococcus neoformans. J. Microbiol. Biotechnol., 27(2), 357-364.

2016

Yi, D.-G., Kim, M. J., Choi, J. E., Lee, J., Jung, J., Huh, W.-K., and Chung, W.-H. (2016). Yap1 and Skn7 genetically interact with Rad51 in response to oxidative stress and DNA double-strand break in Saccharomyces cerevisiae. Free Radic. Biol. Med., 101(), 424-433.

Klionsky, D. J. et al. (2016). Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition). Autophagy, 12(1), 1-222.

2015

Yi, D.-G., and Huh, W.-K. (2015). UDP-glucose pyrophosphorylase Ugp1 is involved in oxidative stress response and long-term survival during stationary phase in Saccharomyces cerevisiae. Biochem. Biophys. Res. Commun., 467(4), 657-663.

Yi, D.-G., and Huh, W.-K. (2015). PKA, PHO and stress response pathways regulate the expression of UDP-glucose pyrophosphorylase through Msn2/4 in budding yeast. FEBS Lett., 589(18), 2409-2416.

Miller, K. E., Kim, Y., Huh, W.-K., and Park, H.-O. (2015). Bimolecular fluorescence complementation (BiFC) analysis: advances and recent applications for genome-wide interaction studies. J. Mol. Biol., 427(11), 2039-2055.

2014

Ha, C. W., Kim, K., Chang, Y. J., Kim, B., and Huh, W.-K. (2014). The β-1,3-glucanosyltransferase Gas1 regulates Sir2-mediated rDNA stability in Saccharomyces cerevisiae. Nucleic Acids Res., 42(13), 8486-8499.

Lee, K., Sung, M.-K., Kim, J., Kim, K., Paik, H., Kim, B., Huh, W.-K., and Ideker, T. (2014). Proteome-wide remodeling of protein location and function by stress. Proc. Natl. Acad. Sci. U.S.A., 111(30), E3157-E3166.

Song, Y. B., Park, C. O, Jeong, J.-Y., and Huh, W.-K. (2014). Monitoring G protein-coupled receptor activation using an adenovirus-based beta-arrestin bimolecular fluorescence complementation assay. Anal. Biochem., 449(), 32-41.

2013

Sung, M.-K., Lim, G., Yi, D.-G., Chang, Y. J., Yang, E. B., Lee, K., and Huh, W.-K. (2013). Genome-wide bimolecular fluorescence complementation analysis of SUMO interactome in yeast. Genome Res., 23(4), 736-746.

2012

Choi, E.-W., Seen, D.-S., Song, Y. B., Son, H.-S., Jung, N.-C., Huh, W.-K., Hahn, J.-S., Kim, K., Jeong, J.-Y., and Lee, T. G. (2012). AdHTS: A high-throughput system for generating recombinant adenoviruses. J. Biotechnol., 162(2-3), 246-252.

Ha, C. W., Sung, M.-K., and Huh, W.-K. (2012). Nsi1 plays a significant role in the silencing of ribosomal DNA in Saccharomyces cerevisiae. Nucleic Acids Res., 40(11), 4892-4903.

Klionsky, D. J. et al. (2012). Guidelines for the use and interpretation of assays for monitoring autophagy. Autophagy, 8(4), 445-544.

2011

Pu, J., Ha, C. W., Zhang, S., Jung, J. P., Huh, W.-K., and Liu, P. (2011). Interactomic study on interaction between lipid droplets and mitochondria. Protein & Cell, 2(6), 487-496.

Shin, C.-S., and Huh, W.-K. (2011). Bidirectional regulation between TORC1 and autophagy in Saccharomyces cerevisiae. Autophagy, 7(8), 854-862.

Ha, C. W., and Huh, W.-K. (2011). The implication of Sir2 in replicative aging and senescence in Saccharomyces cerevisiae. Aging, 3(3), 319-324.

Kim, Y., Chay, K.-O., Song, Y. B., Kim, T.-Y., Kim, I., Han, S.-J., Ahn, Y., Cho, S.-H., Hoe, K.-L., Ahn, B. W., Huh, W.-K., and Lee, S.-R. (2011). Redox regulation of the tumor suppressor PTEN by glutaredoxin 5 and Ycp4. Biochem. Biophys. Res. Commun., 407(1), 175–180.

Lee, P., Paik, S. M., Shin, C. S., Huh, W.-K., and Hahn, J. S. (2011). Regulation of yeast Yak1 kinase by PKA and autophosphorylation-dependent 14-3-3 binding. Mol. Microbiol., 79(3), 633-646.

Ha, C. W., and Huh, W.-K. (2011). Rapamycin increases rDNA stability by enhancing association of Sir2 with rDNA in Saccharomyces cerevisiae. Nucleic Acids Res., 39(4), 1336-1350.

2010

Chang, Y. J., Shin, C.-S., Han, D., Kim, J., Kim, K., Kwon, Y.-M., and Huh, W.-K. (2010). Genomewide profiling of rapamycin sensitivity in Saccharomyces cerevisiae on synthetic medium. Genomics & Informatics, 8(4), 177-184.

Bandyopadhyay, S., Mehta, M., Kuo, D., Sung, M.-K., et al. (2010). Rewiring of genetic networks in response to DNA damage. Science, 330(6009), 1385-1389.

Sung, M.-K., and Huh, W.-K. (2010). In vivo quantification of protein-protein interactions in Saccharomyces cerevisiae using bimolecular fluorescence complementation assay. J. Microbiol. Methods, 83(2), 194–201.

Kim, Y., Song, Y. B., Kim, T.-Y., Kim, I., Han, S.-J., Ahn, Y., Cho, S.-H., Chay, K.-O., Yang, S. Y., Ahn, B. W., Huh, W.-K., and Lee, S.-R. (2010). Redox regulation of the tumor suppressor PTEN by glutathione. FEBS Lett., 584(16), 3550-3556.

허원기, 송용범, 이태규, 신동승, 정재연, 최은욱, 안지선 (2010). 바이러스 벡터를 이용하여 단백질의 상호작용을 측정하는 방법. 대한민국 특허 (출원번호 10-2010-0058213, 출원일자 2010.06.18; 등록번호 10-1029972, 등록일자 2011.04.12), (), .

Song, Y. B., Jhun, M. A., Park, T., and Huh, W.-K. (2010). Quantitative proteomic analysis of ribosomal protein L35b mutant of Saccharomyces cerevisiae. Biochim. Biophys. Acta, 1804(4), 676-683.

2009

Shin, C.-S., Chang, Y.-J., Lee, H.-G., and Huh, W.-K. (2009). Rapamycin-Induced Abundance Changes in the Proteome of Budding Yeast. Genomics & Informatics, 7(), 203-207.

허원기, 성민경, 양은빈, 이대관, 임규범 (2009). 생체 내 단백질 상호작용 분석을 위한 황색형광단백질 N-말단 절편 부착 효모 균주 라이브러리. 대한민국 특허 (출원번호 10-2009-0048746, 출원일자 2009.06.02; 등록번호 10-1139589, 등록일자 2012.04.17), (), .

Shin, C.-S., Kim, S. Y., and Huh, W.-K. (2009). TORC1 Controls Degradation of Transcription Factor Stp1, a Key Effector of the SPS Amino Acid-Sensing Pathway in Saccharomyces cerevisiae. J. Cell Sci., 122(), 2089-2099.

Kim, T.-Y., Ha, C. W., and Huh, W.-K. (2009). Differential Subcellular Localization of Ribosomal Protein L7 Paralogs in Saccharomyces cerevisiae. Mol. Cells, 27(), 539-546.

2008

Lee, K. Y., Chuang, H.-Y., Beyer, A., Sung, M.-K., Huh, W.-K., Lee, B., and Ideker, T. (2008). Protein networks markedly improve prediction of subcellular localization in multiple eukaryotic species. Nucleic Acids Res., 36(), e136.

허원기, 성민경 (2008). 이분자 형광 상보 기법을 이용하여 단백질의 수모화를 분석하는 방법 및 그에 사용되는 벡터들. 대한민국 특허 (출원번호 10-2008-0094298, 출원일자 2008.09.25; 등록번호 10-1050669, 등록일자 2011.07.13), (), .

Huh, W.-K., Song, Y. B., Lee, Y.-S., Ha, C. W., Kim, S.-T., and Kang, S.-O. (2008). D-Erythroascorbic acid activates cyanide-resistant respiration in Candida albicans. Biochem. Biophys. Res. Commun., 369(), 401-406.

Sung, M.-K., Ha, C. W., and Huh, W.-K. (2008). A vector system for efficient and economical switching of C-terminal epitope tags in Saccharomyces cerevisiae. Yeast, 25(), 301-311.

허원기, 성민경 (2008). 에피토프 태그 치환용 재조합 벡터. 대한민국 특허 (출원번호 10-2008-0003443, 출원일자 2008.01.11; 등록번호 10-0927098, 등록일자 2009.11.09), (), .

Lee, H.-G., Lee, H.-S., Jeon, S.-H., Chung, T.-H., Lim, Y.-S., and Huh, W.-K. (2008). High-resolution analysis of condition-specific regulatory modules in Saccharomyces cerevisiae. Genome Biol., 9(), R2.

2007

Sung, M.-K., and Huh, W.-K. (2007). Bimolecular fluorescence complementation analysis system for in vivo detection of protein-protein interaction in Saccharomyces cerevisiae. Yeast, 24(), 767-775.

Lee, M.-W., Kim, B.-J., Choi, H.-K., Ryu, M.-J., Kim, S.-B., Kang, K.-M., Cho, E.-J., Youn, H.-D., Huh, W.-K., and Kim, S.-T. (2007). Global protein expression profiling of budding yeast in response to DNA damage. Yeast, 24(), 145-154.

2006

허원기, 성민경 (2006). 염색체내 형광단백질 유전자 일부가 삽입된 세포를 포함하는 이분자 형광 상보 시스템 및 이를 이용한 이분자 형광 상보 기법. 대한민국 특허 (출원번호 10-2006-0096709, 출원일자 2006.09.30; 등록번호 10-0825290, 등록일자 2008.04.21), (), .

2005

Howson, R., Huh, W.-K., Ghaemmaghami, S., Falvo, J. V., Bower, K., Belle, A., Dephoure, N., Wykoff, D. D., Weissman, J. S., and O’Shea, E. K. (2005). Construction, verification and experimental use of two epitope-tagged collections of budding yeast strains. Comp. Funct. Genom., 6(), 2-16.

2003

Huh, W.-K., Falvo, J., Gerke, L., Carroll, A., Howson, R., Weissman, J. S., and O'Shea, E. K. (2003). Global analysis of protein localization in budding yeast. Nature, 425(), 686-691.

Ghaemmaghami, S., Huh, W.-K., Bower, K., Howson, R., Belle, A., Dephoure, N., O'Shea, E. K., and Weissman, J. S. (2003). Global analysis of protein expression in yeast. Nature, 425(), 737-741.